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Biology
1) Mouse brain
Very typical biological tissues for serial block-face imaging. They have high density of membranes and become highly conductive when stained and yields good contrast between membrane and cytosol.
SEM: Zeiss Sigma VP
Acceleration voltage: 2.5 kV
Voxel size: 12 x 12 x 30 nm
2) Lamprey spinal cord
Samples courtesy of Dr Alan Kuzirian at Marine Biology Laboratory University of Chicago.
SEM: Zeiss Sigma VP
Acceleration voltage: 3 kV
Cut thickness: 40 nm
3) Squid skin
Samples courtesy of Dr Alan Kuzirian at Marine Biology Laboratory University of Chicago.
SEM: Zeiss Sigma VP
Acceleration voltage: 1.9 kV
Cut thickness: 25 nm
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