Biology

1) Mouse brain

Very typical biological tissues for serial block-face imaging. They have high density of membranes and become highly conductive when stained and yields good contrast between membrane and cytosol. 

SEM: Zeiss Sigma VP

Acceleration voltage: 2.5 kV

Voxel size: 12 x 12 x 30 nm

2) Lamprey spinal cord

Samples courtesy of Dr Alan Kuzirian at Marine Biology Laboratory University of Chicago. 

SEM: Zeiss Sigma VP

Acceleration voltage: 3 kV

Cut thickness: 40 nm

3) Squid skin

Samples courtesy of Dr Alan Kuzirian at Marine Biology Laboratory University of Chicago. 

SEM: Zeiss Sigma VP

Acceleration voltage: 1.9 kV

Cut thickness: 25 nm